cell-type deconvolution

SimBu: Bias-aware simulation of bulk RNA-seq data with variable cell type composition

As complex tissues are typically composed of various cell types, deconvolution tools have been developed to computationally infer their cellular composition from bulk RNA sequencing (RNA-seq) data. To comprehensively assess deconvolution performance, …

Mass-spectrometry-based draft of the Arabidopsis proteome

Plants are essential for life and are extremely diverse organisms with unique molecular capabilities1. Here we present a quantitative atlas of the transcriptomes, proteomes and phosphoproteomes of 30 tissues of the model plant Arabidopsis thaliana. …

Quantitative comparison of within-sample heterogeneity scores for DNA methylation data

DNA methylation is an epigenetic mark with important regulatory roles in cellular identity and can be quantified at base resolution using bisulfite sequencing. Most studies are limited to the average DNA methylation levels of individual CpGs and thus …

Comprehensive evaluation of transcriptome-based cell-type quantification methods for immuno-oncology

*Motivation*The composition and density of immune cells in the tumor microenvironment (TME) profoundly influence tumor progression and success of anti-cancer therapies. Flow cytometry, immunohistochemistry staining or single-cell sequencing are often …